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Chemical structure of Nitrotetrazolium Blue chloride

GC15554

Nitrotetrazolium Blue chloride

Also known as 2-[2-methoxy-4-[3-methoxy-4-[3-(4-nitrophenyl)-5-phenyltetrazol-2-ium-2-yl]phenyl]phenyl]-3-(4-nitrophenyl)-5-phenyltetrazol-2-ium dichloride

Nitrotetrazolium Blue chloride (NBT) is a dehydrogenase substrate that is used in western blots and immunohistochemical staining with the alkaline phosphatase (ALP) substrate 5-bromo-4-chloro-3-indolyl phosphate (BCIP) to produce a purple-blue precipitate

ChemicalCAS298-83-9MW817.63FormulaC40H30N10O6 • 2Cl
CAS number
298-83-9
Molecular weight
817.63
Formula
C40H30N10O6 • 2Cl
Solubility
≥ 10mg/mL in Water with gentle warming, 70%DMF ： ≥ 100 mg/mL (122·30 mM), DMSO ： 33·33 mg/mL (40·76 mM)
InChIKey
FSVCQIDHPKZJSO-UHFFFAOYSA-L
Catalogue number
GC15554
Brand
GLPBIO
Computed properties PubChem CID 9281 · TPSA 179 Ų · H-bond donors 0 · H-bond acceptors 12
TPSA
179 Ų
H-bond donors
0
H-bond acceptors
12
Rotatable bonds
9
Heavy atoms
58

SMILES COC1=C(C=CC(=C1)C2=CC(=C(C=C2)[N+]3=NC(=NN3C4=CC=C(C=C4)[N+](=O)[O-])C5=CC=CC=C5)OC)[N+]6=NC(=NN6C7=CC=C(C=C7)[N+](=O)[O-])C8=CC=CC=C8.[Cl-].[Cl-]

Computed descriptors from PubChem (CID 9281), public domain. Identity cross-checked against the supplier's molecular formula and weight — verify before use.

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